Microsampling to monitor conventional and novel biomarkers for pediatric and adult nephrology patients

A two-part presentation exploring the utility of microsampling devices in nephrology patients; along with the optimization of the microsampling workflow.

To begin we will compare microsampling devices with conventional blood and urine collection methods for the monitoring of conventional (and novel) biomarkers in adults with kidney transplantation and glomerulonephritis, along with a discussion relating to the clinical benefits of microsampling. The conversation will then move to the optimization of the laboratory workflow relating to the monitoring and identification of novel protein biomarkers for chronic kidney disease patients from microsamples using proteomic techniques. This will primarily focus on dried blood spots, but other biofluid microsamples will also be discussed.

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What you will learn:

Potential benefits of microsampling in comparison to conventional sampling methods in the nephrology patient population.
Processing methodology for dried blood spots.
Comparisons between the varying dried blood spot devices available.
Identification of biomarkers using microsampling techniques.

Speakers:

Kashif Anwari
PhD Candidate (Salama Lab), Renal/General Medicine Registrar, University College London/Royal Free Hospital (London)

Kashif is a renal registrar doctor based in North London. He has also recently started a PhD under Professor Salama in an effort to define remission in patients with glomerulonephritis by granularly studying blood and urine conventional (and novel) biomarkers. When not seeing patients or performing ELISAs, you can usually find Kashif watching a Liverpool FC game, listening to qawalli/hip-hop....or running after his 2 year old.

Hannah Ging
PhD Student, University of Liverpool

Hannah is a first year PhD student at the University of Liverpool, working in both the Centre for Proteome Research and the Institute in the Park (Alder Hey Children's Hospital). The focus of Hannah's research is on the use of dried biofluids for proteomic analysis of pediatric CKD patients. Before this, Hannah completed a MSci in Biochemistry at Lancaster University.

Moderated by:

Neil Spooner
Founder, PCSIG

Neil is the Founder of Spooner Bioanalytical Solutions, helping companies to integrate biological fluid microsampling, and patient centric sampling and analysis into workflows. He also works with organisations to develop technologies and introduce them to the market. Neil also assists organisations with their understanding of emerging trends in the pharmaceutical industry and bioanalysis. Neil is a Senior Visiting Research Fellow at the University of Hertfordshire, Editor in Chief of the journal, Bioanalysis, Founder of the Patient Centric Sampling Interest Group and Co-Chair of the Reid Bioanalytical Forum. He has published over 70 peer reviewed manuscripts and delivered over 50 podium presentations.​​​​​​​

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